日本和曼氏血吸虫抗原在间接血凝试验中与不同血吸虫病人血清的交叉反应

Cross Reactivity of Schistosoma Japonicum and S. MansoniAntigen Preparations in IHA with Various Schistosomiasis Patient Sera

用间接血凝试验(IHA)比较不同抗原SjSEA、SmAWA和SmBW与日本、曼氏和埃及血吸虫病人血清的交叉反应。结果显示:3种抗原致敏的绵羊红细胞(SRBC)与其相应的血吸虫病人血清反应率分别为100％(SjSEA),96％(SmAWA)和98％(SmBW);与不同种血吸虫病人血清的交叉反应率在79％～95％,其中以SjSEA最高。结果提示:SjSEA除可用于诊断日本血吸虫病外,也可作为诊断曼氏和埃及血吸虫病的候选抗原。此外,本试验还发现IHA抗体滴度与病人排出的虫卵数量无相关关系。

We compared cross reactivities of soluble egg antigen of Schistoso-ma japonicum (SjSEA), crude adult worm antigen of S. mansoni (SmAWA) and partially purified adult worm antigen of S. mansoni (SmBW) in an indirect haemagglutination test (IHA) with sera of patients with S.japonicum. S.mansoni, S.haematobium, respectively. The results showed that sheep red blood cells (SRBC) sensitized with three different antigens were highly sensitive in cases of homolgous schistosomiasis. 100% (SjSEA), 96% (SmAWA) and 98% (SmBW). The rate of cross reactivity with various schistosomiasis sera ranged between 79% and 95%, among which SRBC sensitized with SjSEA was 'highest. These results suggest that besides being used to diagnose schistosomiasis japonicum, SjSEA is also a candidate for diagnosis of schistosomiasis mansoni and haematobium. Therefore, it was also found that there were not correlations between the titres in IHA ard the number of eggs which the patients excreted.