肺癌患者血清IL-6、IL-8和IL-10检测及临床意义

The clinical significance of serum interleukin-6, interleukin-8 and interleukin-10 levels in patients With lung cancer

目的 探讨血清白细胞介素 6 (IL 6 )、白细胞介素 8(IL 8)及白细胞介素 10 (IL 10 )在肺癌免疫、发生、进展、转移和预后中的作用及其临床意义。方法 选取TNMⅡ～Ⅳ期的肺癌患者共 4 7例作为研究对象 ,其中 2 3例患者进行了以铂类药为主的化疗 ,在治疗前及治疗后一月应用双抗体夹心酶联免疫吸附法 (ELISA)测定其血清IL 6、IL 8及IL 10水平。另对 7例死亡患者于临终前检测血清IL 6、IL 8及IL 10水平。同时取 30例健康成人作为对照组。结果 4 7例肺癌患者不同病理类型之组间比较血清三组细胞因子水平无差异 ,但均明显高于正常对照组。三组间比较P <0 0 0 1;肺癌患者血清IL 6、IL 8及IL 10水平随着临床分期的进展而升高 ,有远处转移的晚期患者的血清IL 6、IL 8和IL 10明显高于无转移的早期患者 ;16例化疗有效 ,病情稳定和缓解的患者血清IL 6、IL 8及IL 10水平较化疗前明显下降 ;7例化疗无效、病情进展者血清IL 8、IL 10测无明显下降 ,IL 6进一步升高 ;7例死亡患者临终前血清IL 8水平有持续升高的趋势。结论 IL 6、IL 8和IL 10在肺癌的发生和进展中具有重要作用。血清IL 6、IL 8和IL 10联合检测对肺癌诊断有一定的参考价值 。

Objective To study clinical significance and the relationship between the serum levels of interleukin 6(IL 6), interleukin 8(IL 8) and interleukin 10(IL 10) and disease progression, prognosis in the patients with lung cancer. Methods The serum levels of IL 6, IL 8 and IL 10 were measured in 47 patients with TNM stage Ⅱ,Ⅲ and Ⅳ lung cancer and healthy control by using an enzyme linked immunosorbent assay(ELISA). Resnlts The serum IL 6, IL 8 and IL 10 levels of the patients with lung cancer were significantly higher than those of the healthy control( P <0 001). Serum levels of IL 6, IL 8 and IL 10 were correlated significantly with tumor's TNM stages( P <0.05). And those were not assorciated with tumor's pathologic classification( P >0 05). After the chemotherapy, the serum levels of IL 6, IL 8 and IL 10 were decreased significantly in patients with stable stage and partial remission but increased in IL 6 serum level in patients with progression. Conclusion The measurement of Il 6, IL 8 and IL 10 serum levels in the patients with lung cancer is a reliable, sensitive and easy method. An elevation of IL 6, IL 8 and IL 10 serum level in the patients with lung cancer may serve as an indication of disease progression and an unfavourable prognosis.