青海湖裸鲤和鲤鱼组织乳酸脱氢酶同工酶比较研究

Comparative analysis of lactic dehydrogenase isozymes in serum and tissues of Qinghai-lake naked carps and carps

采用聚丙烯酰胺凝胶电泳法(PAGE)对青海湖裸鲤和鲤鱼的血清及组织乳酸脱氢酶(LDH)同工酶进行了比较研究。结果表明,青海湖裸鲤的血清及组织LDH共表现出LDHF4、LDHA4、LDHA2B2、LDHB4和LDHE45种主要同工酶,而鲤鱼共表现出LDHF4、LDHA4、LDHA2B2和LDHB44种主要同工酶,并且LDH同工酶在种间和种内不同组织中均表现出分布特异性。

The lactic dehydrogenase(LDH) isozymes in serum and various tissues of Qinghai-lake naked carps and carps were comparatively analyzed with polyacrylamidegel electrophoresis.The results showed that in serum and tissues of Qinghai-lake naked carps there were five major isozymes of LDHB_4,LDHA_2B_2,LDHA_4,LDHE_4 and LDHF_4, and in serum and tissues of carps there were four major isozymes of LDHB_4,LDHA_2B_2,LDHA_4 and LDHF_4.The LDH isozymes showed specific distribution in interspecles and intraspecies.