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冷冻保护剂对延边黄牛体外受精卵的影响 被引量:1

Effects of cryoprotectant on Yanbian cattle zygotes fertilyzed in vitro
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摘要 对延边黄牛体外受精卵进行了毒性实验,结果表明,延边黄牛体外受精桑椹胚在0.5M海藻糖(7.6%)和0.5M蔗糖(3.2%)溶液中的存活率差异不显著,早期囊胚在0.5M海藻糖(58.3%)溶液中的存活率显著高于0.5M蔗糖(34.5%)组,在同一处理内早期囊胚的存活率显著高于桑椹胚(P<0.05).以0.5M海藻糖为基础液,分别用0.0(对照组),2.0,2.5,3.0,3.5M甘油处理延边黄牛体外受精早期囊胚,其存活率对照组与不同浓度甘油组之间差异均不显著. The poisonous esperirnerts were operated on Yanbian cattles' zygotes fentilized in vitro.The result indicacled that the survival rate of the zygotes morulas in vitro had no significant differente cultured in 0.5 M mycoie (7.6%)edution and in 0.5 M saccharu solution.
出处 《延边大学农学学报》 2003年第4期279-282,共4页 Agricultural Science Journal of Yanbian University
基金 国家高新技术研究发展计划 (863计划 )资助项目 (2 0 0 1AA2 43 0 51 )
关键词 冷冻保护剂 延边黄牛 体外受精卵 毒性实验 桑椹胚 体外受精 海藻糖 蔗糖 存活率 zygotes of Yanbian cattles fertilized in vitro cryoprotectant developmental stage of embryo
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