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提高长寿花试管苗增殖和生根率的研究 被引量:1

Studies on Propagation and Rooting Rate of Kalanchoe blossfeldian in vitro
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摘要 以长寿花为材料,研究细胞分裂素BA和生长素NAA对带腋芽茎段和茎尖的增殖及IBA、NAA对幼苗生根的影响。试验结果表明,当茎尖和带腋芽茎段在MS+BA0.4mg L+NAA0.1mg L的培养基中培养,有利于芽增殖、分化,其繁殖系数可达5.8;而在培养基1 2MS+IBA0.4mg L中培养,有利于长寿花幼苗生根。 The propagation of shoot-tip and stem with auxillary bud of Kalanchoe blossfeldian,which were used as explants,was studied in MS medium supplemented with BA and NAA. Auxin NAA combined with IBA could promote the growth of roots.The results indicated that monthly propagation coefficient of Kalanchoe blossfeldian could reach 5.8.It was advantageous to the differentiation of the bud when shoot-tip and stem with auxillary bud were cultured in MS medium supplemented with 0.4mg/L BA and 0.2mg/L NAA.The best medium for the growth of root was 1/2MS+IBA 0.4mg/L,which was suitable to not only rooting but also making plantlets strong.
出处 《特产研究》 2003年第4期15-18,共4页 Special Wild Economic Animal and Plant Research
基金 安徽技术师范学院自然科学基金项目资助(2003Y207)
关键词 长寿花 试管苗 增殖 生根率 细胞分裂素 生长素 带腋芽茎段 茎尖 培养基 外植体 Kalanchoe blossfeldian Plant in vitro Propagation Rooting
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