粘虫中毒后体内酯酶同工酶活力变化的研究

A study on the activities of the esterase isozyme in Mythimna separata walker of different poisonous periods

利用聚丙烯酰胺凝胶电泳技术(PAGE),研究了试虫中毒后不同阶段体内酯酶同工酶的变化情况。两种杀虫剂对粘虫体内酯酶同工酶影响的研究表明,中毒试虫体内酯酶同工酶不同程度地被抑制,个别酶带消失,也有新带的出现,在抑制酯酶的程度上,有机磷类高于氨基甲酸酯类。认为试虫中毒后不同时期体内酯酶同工酶的变化并无一定规律可循。

The EST isozyme in it was analysized by using the technique of vertical slab polyacry lamide gel elec-trophoresis. The EST isozyme in it was inhibited in different degrees. Through the study is made on the esterase zymograms of different poisonous periods after applying insecticides to the supplied insect,the EST isozyme in poisonous insect is inhibited in different degrees. Ops are higher than Carbs in the degree of inhibiting EST isozyme. There is not regular pattern on the changes of EST isozyme of different poisonous periods insect.