基因工程菌枯草芽孢杆菌GEB3产生的脂肽类抗生素及其生物活性研究

Lipopeptide Antibiotics Produced by the Engineered Strain Bacillus subtilisGEB3 and Detection of Its Bioactivity

利用MALDI TOF MS技术 ,鉴定了将lpaB3基因转入枯草芽孢杆菌Bacillussubtilis 168菌株所构建的工程菌GEB3产生的脂肽类抗生素种类。结果表明 ,GEB3仅产生表面活性素 (surfactin) 1种脂肽类抗生素。经LC MS分析 ,GEB3产生由 13、14和 15个碳原子的脂肪酸链构成的标准表面活性素变异体 (standardsurfactinisoforms)。生物活性检测表明 。

MALDI-TOF-MS technology was used for identification o f lipopeptide antibiotics produced by GEB3 strain, a derivative of Bacillus su btilis 168 which transformed by lpaB3 gene. The result showed GEB3 produce d only lipopeptide antibiotic surfactin. The analysis by LC-MS demonstrated tha t GEB3 produced standard surfactin isoforms with side chain lengths of 13,14 and 15 carbon atoms. The bioactivity detection of the surfactin indicated that the surfactin produced by GEB3 had inhibition effect to plant pathogens Pyriculari a oryzae and Rhizoctonia solani.