用Glu-B3、Gli-B1和SEC-1b复合引物PCR检测普通小麦1BL/1RS易位系

Identification of 1BL/1RS Translocation via Multiplex PCR Markers of Glu-B3, Gli-B1 and SEC-1bin Common Wheat

利用低分子量 (LMW )麦谷蛋白Glu B3的STS PCR标记、醇溶蛋白Gli B1的SSR标记和黑麦碱SEC 1b的STS PCR标记的复合PCR ,对 10个普通小麦品种、中优 950 7/CA963 2的 91个DH系和 2 8个F2 个体植株 ,进行了1BL/1RS易位系的检测。结果表明 ,中优 950 7/CA963 2的 3 9个DH系和CA963 2、晋麦 45、兰考 2 4、烟农 18、京冬 8等5个品种缺失 2个小麦贮藏蛋白位点的PCR产物 ,而拥有黑麦碱的PCR产物。利用PAGE和ELISA方法 ,对上述品种 (系 )进行了黑麦碱 (secalin)蛋白的检测 ,发现这些品种 (系 )均包含secalin ,与分子标记的检测结果吻合 ,是 1BL/1RS易位系。对 2 8个F2 单株的检测表明 ,该复合标记可以检测出早代

Glu-B3 STS-PCR, Gli-B1 SSR and SEC-1b STS-PCR markers were used for identification of 1BL/1RS translocation in 10 cultivars, 91 double haploid lines and 28 F 2 single plants of Zhongyou9507/CA9 632. The results indicated that both Glu-B3 and Gli-B1loci were absent in 5 cultivars and adva nced lines such as CA9632, Jinmai45, Lankao24, Yannong18, Jingdong8 and 39 doubl ed-haploid lines while SEC-1b was present. Als o, secalins were detected using PAGE and ELISA tests in those lines, which indic ated that all of those lines include 1RS. The results of PAGE and ELISA tests co nsist with that of multiplex PCR. Furthermore, the multiplex PCR results of 28 F 2 plants demonstrated that multiplex PCR could be used to detect homogenous an d heterogeneous 1BL/1RS plants.