吲哚美辛对小鼠肝癌细胞免疫的影响

THE EFFECTS OF INDOMETHACIN ON THE CELLUAR IMMUNITY OF MOUSE WITH HEPATOCARCINOMA.

吲哚美辛是一重要的生物反应调节剂,广泛用于各种肿瘤的实验性研究和临床辅助治疗。本文采用微量肝癌细胞悬液移植法复制了肝内移植型小鼠肝癌模型,并采用此模型观察吲哚美辛对肝癌小鼠细胞免疫的影响。结果:肝癌模型的建立成功率为100％,无自发性消退。吲哚美辛对肝癌小鼠细胞免疫的影响:显示可明显提高宿主外周血中T辅助淋巴细胞,降低T抑制淋巴细胞的水平,使T辅/T抑比值增高,并且增加外周血中T淋巴细胞数。由此表明,吲哚美辛明显增强肝癌宿主细胞免疫,其作用的实现可能与抑制肝癌组织中前列腺素E_2的合成有关。

Indomethacin is one of the important biological response modifiers and has been applied in the experimen-tal studies and clinical adjunctive treatments of tumors. We reproduced the model of hepatocarcinoma through implanting a tiny amount of cell suspension of ascite hepatoma into mouse liver. The model was used to examine the effects of im-domethacin on the cellular immunity of the mouse. The results showed that the establishment of mouse hepatocarcinoma model, succession rate was 100% and no spontaneously regression. Indomethacin evidently improved the decrement of T-helper lymphocytes and mitigated the increase of T-suppressor lymphocytes, and increased the amount of T-Iymphocytes in peripheral blood. The mechanism of indomethacin may be that indomethacin may cut down the level of prostaglandin E2 through suppressing the synthesis of prostaglandin E_2.