摘要
应用SP免疫组化法检测1980～1989年存档的33例胃癌及其癌旁组织石蜡包埋标本的ras p21和p53基因表达情况,并与临床、病理和随访资料进行对比分析。结果显示:胃癌标本的ras p21阳性表达7例(21.21％),p53蛋白的阳性表达11例(33.33％)。ras p21的阳性表达与病理类型、性别、年龄、民族以及肿瘤部位无关,但肿瘤浸润深度及有无淋巴结转移与ras p21阳性表达之间显著相关。生存不足1年组的ras p21表达阳性率(50％)明显高于1～5年组(22.22％)和5年以上组,表明P21蛋白的表达与胃癌的浸润、进展和预后有关,提示ras p21的检测有可能成为胃癌预后指标,并有指导临床治疗的意义。p53蛋白的表达与患者的临床病理状况无显著相关,提示p53基因的改变可能主要发生在癌变早期,对胃癌的发生起关键作用,但并不影响胃癌细胞的生物学行为。p^53蛋白的表达与ras p^21蛋白表达之间无明显关系。
Using SP immunohistochemical staining, ras p21 and p33 geneprotein expression was investigated in 33 paraffin embedded specimens of gastric carcinoma and their paracarcinoma tissue from 1980~1989. The results showed that (1) The pos-itive rates of ras p21 and p53 gene proteins expression as 21. 2% (7/33) and 33. 3% (11/33) in gastric carcinoma respectively, but nearly no expression in paracarcinoma tissue; (2) There was no relationship between the expression of p21 and sex, age, na-tionality of the patients and pathological types of gastric carcinomas. However, statistical investigation showed that expression of p21 was related to the invasiveness, metastasis and survival time ol gastric carcinoma patients after surgical operation results sug-gest that expression of p21 may be a prognostic index and director of clinical therapy to gastric carcinoma; (3)There was no signif-icant correlation between the p53 gene protein expression in gastric carcinoma and clinical and pathological indexes. It suggested that the p53 gene mutation may occur in the early stage of gastric carcinoma and play an important sole in gastric carcinoma gen-eration, whereas its expression did not affect the biologic behavior of gastric carcinoma; (4)There was no significant correlation between the expression s of ras p21 and p53 gene proteins.
出处
《临床肿瘤学杂志》
CAS
1996年第3期32-34,共3页
Chinese Clinical Oncology
