修饰聚合物-盐水液-固亲和萃取体系分离纯化大豆中的氨基酸

Separation and Purification of Amino-Acids in Soya Bean by Modified Polymer Liquid-Solid Affinity Extraction System

用亚氨基二乙酸 (IDA )修饰聚乙二醇 60 0 0 (PEG)后 ,再与 Cu SO4反应 ,形成 PEG修饰聚合物 PEG-(IDA-Cu) 2 ,混合吐温 80、磷酸盐 ,即构成液 -固亲和萃取体系 .采用该体系 ,直接从大豆蛋白匀浆中提取了氨基酸 .选定萃取条件 :磷酸盐摩尔比 n(K2 HPO4)∶ n(Na H2 PO4) =4.8∶ 1,维持体系 p H值 7.70 ,总盐浓度 1.60 mol·L- 1;吐温 80的体积分数 10 .5% .结果表明 :该体系对大豆蛋白匀浆中氨基酸的二次萃取率为 66.5%

The polyethylene glycol-6 000(PEG) modified with iminodiacetic acid(IDA) reacts with CuSO 4 to form the modified polymer complex PEG-(IDA-Cu) 2 .The polymer-salt-H 2O liquid-solid affinity extraction system was composed by P EG -(IDA-Cu) 2, tween80 and phosphate,which was used to directly extract amino -acids from the soya bean homogenate. The optimal conditions are as follows: t he phosphate concentration is 1.6 mol·L -1 ; the mole ratio of K 2HPO 4 to NaH 2PO 4 is 4.8∶1, the pH of system is 7.70 ; the tween80 volum e fraction is 10.5%;the efficiency of two-step extraction is 66.5%.