肾衰康抗肾间质纤维化的实验研究

Experiment study on the antirenal interstitial fibrosis effect of Shenshuaikang liquid(肾衰康)

目的 :探讨中药肾衰康延缓慢性肾衰竭进展的机制。方法 :采用 5 / 6肾切除方法制成大鼠慢性肾衰竭模型 ,并设假手术组。治疗组灌服肾衰康 ,对照组 (病理组 )及假手术组灌服自来水 ,90 d后比较 3组尿素氮(BUN)、血肌酐 (SCr)、血清转化生长因子 β1(TGFβ1)、纤维连接蛋白 (FN)及层黏蛋白 (L N) ,同时观察肾脏病理改变。结果 :治疗组 BU N(11.2 6± 2 .96 ) mm ol/ L 及 SCr(95 .2 6± 7.99) μm ol/ L 均显著低于对照组 BUN(18.33± 3.93) mm ol/ L 及 SCr(12 7.90± 2 3.2 9) μmol/ L(P均 <0 .0 1) ;治疗组 TGFβ1(4 .5 9± 0 .90 ) μg/ L 及FN(9.76± 1.88) mg/ L均低于对照组 TGFβ1(8.16± 3.14 )μg/ L和 FN(32 .11± 12 .5 0 ) mg/ L (P均 <0 .0 1) ;L N(16 .6 7± 9.6 5 )μg/ L亦较对照组 (2 2 .2 7± 6 .80 )μg/ L低 (P<0 .0 5 )。对照组肾间质增宽并有明显的纤维化病变 ;治疗组病变较轻 ,间质无明显纤维化 ,仅少数肾小球节段性系膜增多或节段性硬化。结论 :肾衰康能降低血中 TGFβ1、FN及 L N,减轻肾脏损害 ,抑制间质纤维化 ,从而延缓慢性肾衰竭的进展。

Objective: To investigate the mechanism of traditional Chinese medicine Shenshuaikang liquid(肾衰康) improving chronic renal failure. Methods: A rat model of chronic renal failure was established by 5/6 renal resection, another group was given sham operation. The rats were randomly divided into three groups(the treatment group, the control group,the sham operation group). The treatment group was given Shenshuaikang liquid, the control group and the sham operation group were given water. The content of blood urea nitrogen (BUN), serum creatinine(SCr), transforming growth factorβ1(TGFβ1), fibronectin(FN), laminin (LN) and renal pathological change were observed in the three groups after 90 days. Results: Compared to those in the control group, the concentration of BUN and SCr were lower, they were (11.26± 2.96)mmol/L vs. (18.33±3.93)mmol/L and (95.26±7.99)μmol/L vs. (127.90±23.29)μmol/L (all P<0.01); serum TGFβ1 and FN levels were decreased,they were (4.59±0.90) μg/L vs. (8.16± 3.14) μg/L and (9.76±1.88)mg/L vs. (32.11±12.50)mg/L (all P<0.01); serum concentration of LN reduced, they were (16.67±9.65)μg/L vs. (22.27±6.80)μg/L (P<0.05). Renal interstitial in the control group increased and complicated with significant fibrosis. On the contrary the renal pathological change was slight in the treatment group,renal interstitial had no fibrosis but a few glomeruli segmental mesangial proliferation and segamental sclerosis. Conclusion: Shenshuaikang liquid can decrease the serum concentration of TGFβ1, FN and LN, and then ameliorate the renal damage. It can also inhibit interstitial fibrosis and prevent the progress of chronic renal failure.