摘要
目的用柔嫩艾美耳球虫(Eimeriatenella)孢子表面蛋白基因在大肠杆菌中的表达产物免疫雏鸡,观察其诱导产生的免疫应答反应和对球虫攻击的保护性作用。方法重组抗原经肌肉注射菌体蛋白和口服工程活菌分别于7、14、28日龄3次免疫海兰小公雏,同时设菌体蛋白+小剂量活卵囊组、未免疫攻毒组、未免疫未攻毒组作对照,采用间接ELISA法、流式细胞仪技术检测鸡体产生的免疫应答反应。于末次免疫后2w(42日龄)用5×104个E.tenella卵囊进行攻毒,观察其诱导产生的保护力。结果口服工程活菌组无论从卵囊记数、盲肠病变记分、相对增重、抗体产生水平以及细胞免疫水平上均优于其它免疫组。结论λMzp5-7基因在大肠杆菌中的表达产物对鸡柔嫩艾美耳球虫的攻击有一定的免疫保护作用。
Objective To observe the immunoprotective effect of the recombinant antigen of λMzp5 7 to chicken challenged with E.tenella oocysts. Methods The chicken were immunized with recombinant antigen three times, then were challenged with E.tenella oocysts. The responses of specific humoral and cell immunity were elevated by the immunological methods such as the specific antibodies responses, the ratio of CD4+/CD8+.Results The E.coli expressing E.tenella recombinant antigen can induce the immune response. At the same time,the results showed immunization with live E.coli expressing E.tenella recombinant antigen can protect against E.tenella infection partial. Oocyst number and time shedding of experiment groups were shorter, and the increase of body weight(BW) were swifer, and the cecum lesion were slighter than those of control groups. Conclusion The recombinant antigen of λMzp5 7 showed an immunoprotective effect to chicken challenged with E.tenella oocysts.
出处
《热带医学杂志》
CAS
2003年第4期407-410,共4页
Journal of Tropical Medicine
基金
国家自然科学基金农业倾斜项目(No.30170696)。
