摘要
A strain of hydrogen producing bacteria was immobilized by polyvinyl alcohol-boric acid method, with the addition of a small amount of calcium alginate. The immobilized cells were insensitive to the presence of traces of O2. Moreover, the immobilized cells increased both the evolution rate and the yield of hydrogen production. Batch experiments with a medium containing 10 g/L glucose demonstrated the yields of hydrogen production by the immobilized and free cells were 2.14 mol/mol glucose and 1.69 mol/mol glucose, respectively. In continuous cultures at medium retention time of 2.0 h, the yield and the evolution rate of hydrogen production by the immobilized cells were 2.31 mol/mol glucose and 1 435.4 ml/（L·h） respectively. However, at medium retention time of 6.0 h, the yield and the evolution rate of hydrogen production by free cells were only 1.75 mol/mol glucose and 362.9 ml/（L·h）, respectively.
A strain of hydrogen producing bacteria was immobilized by polyvinyl alcohol-boric acid method, with the addition of a small amount of calcium alginate. The immobilized cells were insensitive to the presence of traces of O_2. Moreover, the immobilized cells increased both the evolution rate and the yield of hydrogen production. Batch experiments with a medium containing 10 g/L glucose demonstrated the yields of hydrogen production by the immobilized and free cells were 2.14 mol/mol glucose and 1.69 mol/mol glucose, respectively. In continuous cultures at medium retention time of 2.0 h, the yield and the evolution rate of hydrogen production by the immobilized cells were 2.31 mol/mol glucose and 1 435.4 ml/(L·h) respectively. However, at medium retention time of 6.0 h, the yield and the evolution rate of hydrogen production by free cells were only 1.75 mol/mol glucose and 362.9 ml/(L·h), respectively.
基金
SponsoredbytheNational973ProjectofChina(GrantNo .G2 0 0 0 0 2 64 0 2 ) .
关键词
氢气生产
藻酸钙
氧气
葡萄糖
二氧化碳
环境污染
水处理
hydrogen producing bacteria
immobilized cells
characteristics of hydrogen production
