归元片对小鼠吗啡行为敏化的影响

THE EFFECTS OF GUIYUAN TABLETS ON MORPHINE-INDUCED BEHAVIORAL SENSITIZATION IN MICE

目的 :探讨中药复方制剂归元片对吗啡行为敏化的影响。方法 :测定小鼠的自主活动 ,观察归元片对小鼠自主活动的影响。给小鼠慢性吗啡处理 ,建立吗啡诱导的行为敏化小鼠模型 ,观察归元片对吗啡行为敏化的影响。结果 :(1)归元片 (2 5 - 10g·kg- 1 )明显抑制小鼠的自主活动 ,并呈剂量依赖性 ;(2 )归元片剂量依赖性地阻断小鼠吗啡行为敏化的获得和表达 ,但对行为敏化的转换无影响。结论 :归元片对中枢神经系统具有抑制作用 ,可以阻止吗啡成瘾行为的形成 ,对吗啡诱导的敏化行为具有抑制作用。

Objective: To investigate the effects of Guiyuan tablets, a composite of traditional Chinese medicine, on morphine-induced sensitization. Methods: Locomotor activity was measured every 5 minutes for 1 hour immediately after administration with Guiyuan tablets in mice. Mice were made behavioral sensitization to morphine by subcutaneous injection of 20 mg·kg -1 morphine once daily for 7 days, and treated with the combination of morphine (sc) and Guiyuan tablets (ig) to investigate the effects of Guiyuan tablets on the sensitization to morphine. Results: (1) Guiyuan tablets dose-dependently inhibited the locomotor activity in mice; (2) Guiyuan tablets obviously blocked the development and expression of behavioral sensitization to morphine in mice, but did not affect the transfer of behavioral sensitization. Conclusion: Guiyuan tablets have inhibitory effects on central nervous system and suppress the development of morphine addiction behavior. These findings indicate that Guiyuan tablets may be of interferential potential to psychological dependence on morphine in clinic.