摘要
目的 :比较成人卵巢、胎儿卵巢免疫原性的差异 ,以及培养前后胎儿卵巢免疫原性的变化。方法 :对成人及培养前后胎儿卵巢的冰冻切片用HLA -ABC及HLA -DR单克隆抗体进行免疫组织化学染色 ,观察其阳性细胞形态及分布并计数。结果 :成人卵巢内HLA -ABC及HLA -DR抗原含量显著多于胎儿卵巢 (P <0 0 0 1) ,且培养后胎儿卵巢HLA -DR抗原含量显著低于培养前 (P <0 0 1) ,随培养天数的增加HLA -DR抗原含量逐渐减少 (P <0 0 1)。结论 :胎儿卵巢的免疫原性低于成人卵巢 ,且培养后进一步降低。以培养一段时间的胎儿卵巢作为移植供体 ,必将提高临床卵巢移植的成功率。
Objective:To compare the immunogenicity of adult ovary,uncultured and cultured fetal ovary.Methods:To observe the expression of HLA-ABC and HCA-DR antigens by using immunohistochemical method on the frozen sections of adult and fetal ovary.Results:The HLA-ABC and HLA-DR antigens in adult ovary were significantly higher than that in.The HLA-DR antigen in cultured fetal ovary were significantly higher than that in unculturedone,and they decreased following with the increase of cultured days.Conclusions:The immunogenicity of fetal ovary is lower than that of adult ovary,and reduce after being cultured.As a graft of ovary transplantation,the cultured fetal ovary may prolong the survival rate of grafts.
出处
《内蒙古医学杂志》
2003年第6期471-474,共4页
Inner Mongolia Medical Journal
