3-氯-1,2-丙二醇在大鼠体内的吸收、分布和排泄

Study on the absorption,distribution and excretion of 3-chloro-1,2-propandiol in rats

目的 探讨经口给 3 氯 1,2 丙二醇 (3 MCPD)在大鼠体内的吸收、分布和排泄状况。方法 选用健康雄性成年SD大鼠 ,每个时相点 3只动物。采用毛细管GC MS的方法测定大鼠血液、肝脏、肾脏、大脑和睾丸中的 3 MCPD含量以及 3 MCPD经胆汁、粪便和尿液的排泄。结果 一次性经口给药 75mg/kgBW 2h后在血液、睾丸和肾脏中 3 MCPD的浓度分别为 (6 7 4 6± 7 72 ) μg/g ,(78 37± 5 15 ) μg/ g和 (5 6 2 1± 3 6 4 ) μg/ g ;给药 2 4h后这 3种组织中 3 MCPD的含量分别为(1 0 7± 0 97) μg/g ,(4 9 4 3± 2 8 18) μg/ g和 (11 4 1± 2 5 5 ) μg/g。 2 4h内经尿排出的 3 MCPD原形化合物占总给药量的 (9 74± 3 0 5 ) %。结论 研究结果提示 3 MCPD经胃肠道吸收快 ,迅速和广泛地分布于体内各组织中 ,在靶器官中具有蓄积性 ,3 MCPD原形物主要是通过肾脏排出体外。推测3 MCPD主要经过肝脏代谢。

Objective To explore the absorption,distribution and excretion of 3-Chloro-1,2-propandiol (3-MCPD) in healthy male SD rats after oral administration. Methods 3-MCPD was administrated with a single oral dosage of 75 mg/kg BW to each rat. Samples of blood,tissues (including liver,kidney,brain and testicle) and excreta were then collected,and analyzed by the GC-MS method to determine 3-MCPD concentrations. The reported value is the mean value of three rats. Results At 2 h after the administration,3-MCPD concentrations in blood,testicle and kidney were (67.46±7.72),(78.37±5.15)and (56.21±3.64)μg/g,respectively. At 24 h,however,the corresponding values changed to (1.07±0.97) μg/g,(49.43±28.18)μg/g and (11.41±2.55)μg/g. During the 24-hour period,9.74 ± 3.05 % of the given parent compound was excreted in urine,whereas 0.56 ± 0.22 % and 0.28 ± 0.03 % were excreted in feces and bile,respectively,which implies that kidney is a major organ for excretion 3-MCPD. Conclusions 3-MCPD was quickly absorbed through the alimentary tract and quickly distributed into a number of tissues,and then accumulated in the target organs,especially in the testicle. The excretion of the parent compound was largely through the kidney. It was inferred that 3-MCPD was mainly metabolized in the liver.