摘要
探讨脐血CIK细胞对K5 6 2细胞的杀伤活性及诱导凋亡作用。通过第 1天在脐血淋巴细胞中加入IFN r,第 2天再加入IL 2和CD3单抗进行细胞培养获得多种细胞因子诱导的杀伤细胞 (Cytokinesinducedkillercells)即脐血CIK细胞。用MTT法测定其抗肿瘤活性 ,原位末端标记法进行凋亡分析 ,台盼蓝拒染法计数细胞。脐血CIK细胞及培养上清抗K5 6 2活性明显优于CD3AK细胞 ,短期培养后其增殖活性低于CD3AK细胞 ;但是加入G CSF可以提高CIK细胞的增殖活性 ,且CIK细胞的抗K5 6 2活性仍高于CD3AK细胞。CIK细胞诱导K5 6 2细胞凋亡率大于CD3AK细胞 ,G CSF能部分抑制CIK细胞诱导的K5 6 2细胞的凋亡。脐血CIK细胞是一种有效的杀伤活化细胞 。
To determine the antitumor(K562)activity of cord blood CIK cells and explore whether cord blood CIK cells could induce K562 cells apoptosis in vitro and the effect of G-CSF on cord blood CIK cells .We obtained cytotoxic effector cells from umbilical cord blood by addition of interferon-γ、interleukin-2(IL-2)and the monoclonal antibody CD3. These cells are termed cytokine-induced killer (CIK) cells. MTT was used to determine the antitumor activity of cord blood CIK cells .Apoptosis was detected by TDT end labelling technique. The antitumor activity of CIK cells and of the supernatants were stronger than CD3AK cells .The ratio is higher than that indued by CD3AK cells .Low dosage G-CSF partially inhibited the effect of CIK cells and elevated the proliferation activity of CIK cells. Cord blood CIK cells have cytotoxicity in vitro against K562 cells.
出处
《基础医学与临床》
CSCD
北大核心
2003年第6期666-669,共4页
Basic and Clinical Medicine
