运用RAPD技术对棺头蟋属昆虫亲缘关系的研究(直翅目:蟋蟀总科,蟋蟀科)

Analysis of relationships among species in the genus Loxoblemmus (Orthoptera: Grylloidea, Gryllidae) using random amplified polymorphic DNA(RAPD)

用RAPD技术研究了棺头蟋属Loxoblemmus 9个种的亲缘关系。研究中每种使用了 3个标本 ,试验所用的 5 4种随机引物中 ,有 9种引物能扩增出清晰而稳定的多态性片断 ,多态性片断共计 193条。根据扩增结果 ,计算了个体间及种间扩增片断共享度和遗传距离 ,用UPGMA法进行聚类分析 ,构建系统树。每个种均各自聚为一类 。

The technique of random amplified polymorphic DNA (RAPD) was used to study the relationships of 9 species in the genus Loxoblemmus. A total of 27 samples were used in this study: 3 samples for each species. Among 54 arbitrary primers (10 bp) under predetermined optimal reaction conditions, 9 primers were informative and yielded a total of 193 clear and reproducible bands. Dendrograms were constructed based on genetic distance by the UPGMA method. Individuals of the same species clustered together. The relationships indicated by cluster analysis was mostly the same as those based on comparative morphology.