摘要
采用RAPD方法测定分析了我国麦红吸浆虫Sitodiplosismosellana (Gehin) 10个地理种群的遗传结构。结果表明 :5种引物在 10个种群中共产生了 32 6个RAPD标记 ,各地理种群之间没有产生各自的特征标记 ;不同种群间的遗传相似程度与其地理间距呈反比 ;种群内的遗传相似程度比种群间的高 ;
Ten geographic populations of the orange wheat blossom midge, Sitodiplosis mosellana (Gehin) (Diptera: Cecidomyiidae) from all wheat grown area in China were investigated by RAPD PCR to determine their genetic structure. Samples of 10 adults were examined for each population. Five selected primers, S18 CCACAGCAGT, S32 TCGGCGATAG, S83 GAGCCCTCCA, S169 TGGAGAGCAG and S250 ACCTCGGCAC, produced a total of 326 RAPD markers. No specific band was observed for every population. The distance analysis shows that similarity within a geographic population is higher than that among different populations; the similarity index ( S xy ) among the populations is inversely proportional to the direct distance among them. Cluster analysis by UPGMA shows that the 10 geographic populations could be gathered into two big groups: spring wheat region group and winter wheat region group.
出处
《昆虫学报》
CAS
CSCD
北大核心
2003年第6期783-787,共5页
Acta Entomologica Sinica
基金
西北农林科技大学植保资源和病虫害治理教育部重点实验室访问学者项目
