白细胞介素1α基因启动子-889位点C/T基因型与冠心病严重程度的关系

The correlation between genotypes at the position-889C/T in the promoter of interleukin-1 alpha and the severity of coronary heart disease

目的 探讨白细胞介素 1α(IL 1α)基因启动子 889位点C/T基因型与冠心病 (CHD)严重程度的相关关系。方法 采用聚合酶链反应 限制性片段长度多态性 (PCR RFLP)分析的方法 ,检测118例CHD患者和 184名健康者IL 1α( 889C/T)基因型 ,并用酶联免疫吸附试验检测了所有研究对象血清IL 1α水平。结果 IL 1α( 889)位点各基因型在心肌梗死组和对照组间的分布差异存在显著性(χ2 =5 96 ,P <0 0 1) ,CT基因型患心肌梗死的相对风险度约是CC基因型的 2 39倍 [比值比 (OR) =2 39,95 %可信区间 (CI) =1 17～ 4 86 ];在CHD组 ,CT基因型患者血清IL 1α水平 (13 5 1± 6 85 )ng/L显著高于CC基因型患者 (8 0 4± 4 4 7)ng/L ,P <0 0 0 1。结论 IL 1α( 889)位点CT基因型与CHD的严重程度存在相关关系 ,其机理可能是该位点DNA突变影响了IL 1α的分泌。

Objective To investigate the correlation between the different genotypes at the position -889 in the promoter of interleukin-1 alpha(IL-1α) and the severity of coronary heart disease(CHD). Methods The genotypes of IL-1α(-889C/T)in 118 CHD patients and 184 healthy controls were analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), and The serum level of IL-1α was detected by enzyme-linked immunoabsorbent assay. Results The distribution of IL-1α (-889C/T) genotypes between myocardial infarction(MI) patients and healthy controls was significantly different (χ 2=5.96, P <0.01). CT or TT genotype carriers were at increased risk with an odds ratio of 2.39 for MI(OR=2.39, 95%CI=1.17～4.86). In CHD group, the IL-1α level in patients with CT genotype (13.51±6.85) ng/L was significantly higher than those with CC genotype[(8.04±4.47) ng/L; P <0.001].Conclusion The CT genotype at position -889 in IL-1α is associated with the severity of CHD, and the DNA mutation at this position will probably affect the secretion of IL-1α.