HEV-IV型感染猕猴的血清学及肝脏组织学的动态变化

Dynamic changes of serum and hepatic histopathology in Rhesus macaque infected by HEV-Ⅳ

目的观察戊型肝炎病毒IV基因型(HEV-IV)静脉接种猕猴后其粪便、血清学及肝脏组织学的动态变化。方法用逆转录PCR检测实验动物接种前、后粪便悬液及血清HEV RNA,用酶联免疫法(ELJSA)检测血清抗HEV IgM及抗HEV IgG,并同步观察其血生化及肝脏组织学的变化。结果接种后第30天2只实验动物均出现明显消化道症状,粪便及血清中均检出HEV RNA,随之ALT、AST同步升高,血清抗HEV IgM和IgG显现阳性,肝脏组织学也相继出现不同程度病理改变。发病后第60天各项生化指标恢复正常。结论云南猕猴是HEV-IV感染的较好的动物模型。

ObjectⅣe To observe the dynamic changes of fecal, serum and hepatic histopathology of experimentally infected Rhesus monkeys with HEV-Ⅳ. Methods A sensitⅣe specific RT-PCR assay was used for the detection of HEV RNA in serum and stool suspensions of experimentally infected Rhesus monkeys. ELISA was used for the detection of anti-HEV IgM and IgG in serum. The changes of serum biochemistry and lⅣer histopathology were observed synchro-nizedly. Results The two experimental animals were both ill with obvious intestinal symptoms 30 days after inoculation and HEV RNA in feces and serum became positⅣe. Serum anti-HEV IgM and IgG conversed to positⅣe with accompany of the elevated levels of ALT and AST. LⅣer biopsy showed different histopathological changes. Biochemistry parameters became normal 60 days after infection. Conclusion Yunan Rhesus monkey is suitable for establishment of experiental animal model of HEV-Ⅳ.