广玉兰的离体培养研究

Invitro Culture of Magnolia grandiflora

以广玉兰的叶芽、花托、花被、雄蕊、幼叶为试材 ,用 MS为基本培养基 ,添加不同浓度的 2 ,4 - D,NAA,6 -BA,筛选适合其脱分化、分化、生根的培养基 ,结果表明 :最适外植体为叶芽 ,在 MS+2 ,4 - D 3.5～ 5 mg/ L +NAA3～ 5 mg/ L +6 - BA0 .8～ 1.2 mg/ L +AC0 .8g/ L 的培养基上都能很好地诱导出愈伤组织 ,其中速率最快的最佳培养基是 MS+2 ,4 - D4 .5 mg/ L+NAA3m g/ L+6 - BA1.2 mg/ L+AC0 .8g/ L;愈伤组织继代培养基为 MS+2 ,4 - D 2 .5 mg/ L +NAA 2 .5 m g/ L +6 - BA 1.2 mg/ L +AC 0 .8g/ L ;胚状体的诱导培养基为 MS+2 ,4 - D 1.5 mg/ L+NAA 1.5 m g/ L+6 - BA2 mg/ L+AC0 .8g/ L;诱导胚状体成苗培养基为 MS+2 ,4 - D0 .2 mg/ L+NAA0 .2 m g/ L +6 - BA2 mg/ L +AC0 .8g/ L;生根培养基为 1/ 2 MS+NAA0～ 1mg/ L +6 - BA1.5～ 2 mg/ L +AC0 .8g/ L .

With leaf bud, receptacle,perianth,stamen,young leaf as material and MS as basic culture which adds different concentration of 2,4-D,NAA,6-BA,The authors attempt to screen optimum culture medium which suit for dedifferentiation,differentiation and shoot division.Results show that the most suitable explants are leaf buds.The medium which can induce callus smoothly is MS+2,4-D 3.5~5 mg/L +NAA 3~5 mg/L +6-BA 0.8~1.2 mg/L +AC 0.8 g/L.Among them the fastest and best medium is MS+2,4-D 4.5 mg/L +NAA 3 mg/L +6-BA 1.2 mg/L +AC 0.8 g/L. The best medium for proliferating is MS+2,4-D 2.5 mg/L+NAA 2.5 mg/L +6-BA 1.2 mg/L+AC 0.8 g/L. The medium for induce embryoid is MS+2,4-D 1.5 mg/L +NAA 1.5 mg/L +6-BA 2 mg/L +AC 0.8 g/L. The medium for induce plant is MS+2,4-D 0.2 mg/L +NAA 0.2 mg/L +6-BA 2 mg/L +AC 0.8 g/L.The rooting medium is 1/2 MS+NAA 0~0.5 mg/L +6-BA 1.5～2 mg/L+AC 0.8 g/L. This research provides reference for the industrialized breeding of Magnolia grandiflora and the entry of other gene.