DNA与表面活性剂相互作用研究(Ⅱ)——基于构象变化的分子水平研究

Studies on the Interaction between DNA and Surfactant(II) ——Studies at Molecular Level Based on Conformational Transition

综述了DNA在稀溶液中与表面活性剂在分子水平上的相互作用的研究进展。介绍了DNA与表面活性剂的两阶段键合过程以及伴生的DNA由线圈向小球的构象变化及其理论解释。同时阐述了相关的对其微观构象变化的研究方法 ,如热变性实验、荧光光谱、荧光显微技术、动静态光散射等。对DNA与表面活性剂形成的复合物作为基因转染载体的前景及限制因素作了简要介绍。

The progress in the study of the interaction between DNA and surfactant in dilute solution was reviewed.The interaction leads to a two-stage binding process accompanied by the conformational transition of DNA from elongated coil to compact globule.The corresponding theoretical explanation was presented.The relative methods used in the study of the DNA's conformational transition were introduced,including heating-denature test,fluorescent spectrum,fluorescent microscopic technology and dynamic and static light scatterings etc.Besides,the perspective as well as the limiting elements of the complex formed by DNA and surfactant as the carrier of controlled genetic transfer was discussed briefly.