青岛文昌鱼铁蛋白ferritin基因的序列分析、同源性比较及二级结构预测

Seqeunce analysis, homology comparision and secondary structure prediction of ferritin protein gene in Qingdao amphioxus

对青岛文昌鱼神经胚cDNA文库进行系统测序 ,分析测序结果 ,获得 4个铁蛋白ferritin的EST ,经过拼接得到含有完整读框的文昌鱼铁蛋白ferritin基因cDNA序列并据此演绎出其氨基酸序列 ,对演绎的文昌鱼铁蛋白ferritin的一级结构进行了分析 ,对其二级结构进行了预测 ,并与多种真核生物中的铁蛋白ferritin进行同源性比较 ,为认识文昌鱼铁蛋白ferritin和研究该分子的进化和功能提供了资料 .

By sequencing the Qingdao amphioxus neurulae cDNA library, We obtained ferritin gene and deduced it's amino acid sequence. We analysed it's frame and compared it with other animal's ferritin protein. The identity with human,dog, Ornithodoros moubata, bullfrog, Danio rerio, C.elegans was 67%, 61%, 67%, 62%, 61% ,60%respectively. The results showed that ferritin gene conserved in evolution and ferritin protein was closer to some vertebrate's ferritin.