摘要
在建立康乃馨从愈伤组织到完整植株的高频再生体系的基础上,用花特异表达启动子驱动的康乃馨ACC氧化酶反义基因通过农杆菌介导法对康乃馨进行遗传转化,获得了3株转基因植株,经多重PCR及PCR-Southem杂交检测,证实康乃馨ACC氧化酶的反义基因已整合进康乃馨的基因组中。
The high frequency regeneration system of carnation from leaf to callus to plandets was established. Then genetic transformation of the carnation with the ACO antisense gene, which controlled by flower-specific expression promoter (PchsA) was performed via agrobacterium mediated method and obtained 3 of transgenic plants via the pathway of regeneration plandets from callus at last. The results of the PCR and PCR-Southern hybridization analysis showed that the carnation ACC oxidase antisense gene was integrated into carnation genomes. It will be a good foundation for improvement the longevity of carnation flowers.
出处
《园艺学报》
CAS
CSCD
北大核心
2003年第6期699-702,共4页
Acta Horticulturae Sinica
基金
中国博士后科学基金项目(200200003M)
云南省自然科学基金项目(2002C0002M)
