水牛急性实验感染肝片吸虫对安替比林代谢动力学的影响

The Effects of Acute Infection of Fasciola hepatica on the Metabolism and Pharmacokinetics of Antipyrine in Water Buffaloes

5头健康雄性去势水牛(2～3岁、体重300～500 kg),经粪便和Dot-ELISA检测确认无肝片吸虫感染。每头水牛一次经口感染1 600个肝片吸虫囊蚴,研究急性感染(一次大剂量)肝片吸虫对水牛安替比林代谢动力学影响。用HPLC法测定血浆安替比林(AP)及其代谢物的浓度,分析其动力学参数。每周定时采血测定血清酶水平变化。结果表明:水牛急性感染肝片吸虫后急性期安替比林静脉给药后的动力学参数没有显著变化,在慢性期,血浆消除半衰期T1/2β延长41.42％,总消除率CL下降60.10％,表观分布容积Vdss减小43.61％,平均保留时间MRT上升41.16％,血浆浓度-时间曲线下面积AUC增大150.61％。Ap给药后48 h内各代谢物的形成比率及尿清除率与对照期相比在急性期无显著差异,而慢性期极显著降低,AP试验结果与血浆酶水平变化相一致。

Five healthy male testictomy water buffaloes of 2-3 years old with 300-500 kg body weight and no fasciolosis confirmed by fecal examination and Dot-ELISA were fed. Each one was dosed orally once 1 600 Fasciola hepatica metacercariae to evaluate the effects of acute infection on antipyrine metabolism. The concentrations of antipyrine in plasma and its metabolites in urine were determined by HPLC. The results showed that no obvious changes of antipyrine kinetics pamameters in acute period, T1/2β 41. 42% longer,CL 60. 10% lower. Vdss down 43. 61%,MRT up 41. 16%, AUC up 150.61% were found,similar result was observed in AP plasma clearance and its metabolites clearance in urine.