地西泮干预幼鼠癫痫持续状态对海马结构的远期保护作用

Study on long term protective effect of diazepam interferential treatme nt on hippocampal formation in immature rats with status epilepticus

目的 探讨幼鼠早期癫痫持续状态 (SE)采用地西泮预防干预治疗对成鼠后海马结构损伤的远期保护作用。方法 健康生后 15～ 2 0d的Wistar幼鼠 72只 ,随机分为生理盐水对照组、地西泮干预组和氯化锂 匹罗卡品腹腔注射诱导的癫痫持续状态组 ,并持续追踪至成鼠阶段 ,应用常规病理及电镜观察海马结构的形态学改变 ,同时应用Timm组织化学染色方法进行苔藓纤维发芽的研究。结果 约 2 / 3的SE幼鼠发育至成鼠阶段后 ,在海马结构的神经元仍可见到变性和坏死性改变 ,以CA1区、CA3区和齿状回为重。Timm染色见CA3区有苔藓纤维发芽现象。约 1/ 3的SE幼鼠发育至成鼠后未见到海马结构的损伤性改变。生理盐水对照组和地西泮干预组均未见海马结构损伤性改变以及苔藓纤维发芽现象。

OBJECTIVE: To study the long term protectice effect of diazepam interferential treatment on hippocampal formation in immature rats with status epilepticus. METHODS: Healthy postnatal Wistar rat pups(n = 72) were divided into control group of normal saline, diazepam interferential group and Lithium-pilocarpine-induced status epilepticus group randomly. They were followed up to the stage of mature rats. Conventional histopathological method and electron microscope were utilized to observe morphological changes in hippocampal formation. In addition, Timm histochemical technique was adopted to study mossy fiber sprouting. RESULTS: About 2/3 of rats in status epilepticu group developed hippocampal neuroal degeneration and necrosis, especially in subfields CA1, CA3 and dentate gyrus. Mossy fiber sprouting was found in the CA3 subfield. There were about 1/3 SE rats without injuries in hippocampal formation. No hippocampus lesions and mossy fiber sprouting were found in both of the normal saline and diazepam interferential groups. CONCLUSION: The damage of hippocampal formation caused by status epilepticus in immature rats can be prevented by diazepam interferential treatment. This protective effect can last to maturity.