黄腹角雉精液的低温保存

PRESERVE THE SEMEN OF CABOT’S TRAGOPAN AT LOW TEMPERATURE

1999— 2 0 0 3年从 17只笼养黄腹角雉 (Tragopancaboti)采集精液 ,稀释后分别保存于 4℃的冰箱和 - 196℃液氮中 .在 4℃条件下 ,保存于生理盐水、C 2液、Lake液、Beltsville液等不同稀释液中的精液 ,其精子存活状况相差很大 ,其中保存于Beltsville液中的精子存活率 (S)最高 ,4 8h后仍有 6 0 %以上的活精子 .在精液的冷冻保存方面 ,受降温过程中精子内外渗透压的变化和结晶的影响 ,且对于冷冻保护剂 (二甲基亚砜 ,DMSO)的体积分数 (φ)以及降温速度的选择非常重要 :DMSO的 φ过高 (如 10 % )或过低 (如 1% ) ,都会大幅降低冷冻后精子的S ,实验发现 φ为 4 %的DMSO最适于黄腹角雉精液的冷冻保存 ;以不同的速度降温 ,也显著地影响着精液的保存效果 (P <0 .0 5 ) .

From 1999 to 2003, the semen of Cabot’s Tragopan( Tragopan caboti ), which was taken from seventeen individuals in captivity, were preserved at 4 ℃ in refrigerator and at -196 ℃ in liquid nitrogen. It is found that the survival rate of the spermatozoa is different in distinct dilution, such as 0.9% salt liquid, C 2 liquid, Lake liquid and Beltsville liquid at 4 ℃. The best dilution is Beltsville liquid, which has more than 50% survival spermatozoa after 48 hours. And the worst is salt liquid, in which the spermatozoa will die out in 36 hours. If one wanted to keep the semen for a long time, cryopreservation is a good way. However, enough attention must be given to following questions during the freeze process: Though DMSO is necessary during the semen frozen, it still has strong side effect. Therefore, it is very important to select feasible concentration of DMSO. Optimal rate for cooling the semen is also important to keep high survival rate.