Phosphoramidon对实验性大鼠心肌损伤的保护作用

Protective effects of phosphoramidon on the experimental myocardial injury in rats

目的 :探讨内皮素转化酶抑制剂 (phosphoramidon)对实验性大鼠心肌损伤的保护作用。方法 :36只Wistar大鼠随机分为 :对照组、损伤组、phosphoramidon保护组 ;2 4h后检测血清肌酸磷酸激酶 (CPK)、乳酸脱氢酶(LDH)活性及血浆内皮素 (ET)含量 ;取心尖部心肌进行病理分级。结果 :损伤组CPK ,LDH活性 ,ET含量均高于对照组和保护组 (P <0 .0 5 ) ;损伤组心肌病理分级高于对照组 (P <0 .0 1) ,保护组分级低于损伤组 (P <0 .0 5 )。结论 :内皮素参与实验性大鼠心肌损伤的发病过程 ;phophoramidon对大鼠损伤心肌有保护作用 ,其机制为抑制内皮素转化酶而减少内皮素产生。

Objective To investigate the protective effects of phosphoramidon on the experimental myocardial injury in rats. Methods Thirty-six Wistar rats were randomly divided into 3 groups of 12: the control group (Control), the isoproterenol group (ISO) and the protection group (PA). All rats were anesthetized after 24 hours and the blood was sampled from the carotid artery. The activities of creatine phosphokinase (CPK) and lactic dehydrogenase (LDH) in the serum and the contents of ET in the plasma were detected; then the hearts were isolated and the total layers of apical myocardium were solidified, embedded, sectioned, stained with HE and graded pathologically. Results The serum activities of CPK and LDH, and the plasmatic content of ET in the ISO group were significantly higher than those in the control group and PA group (P<0.05); the degree of myocardial pathological damage in the ISO group was significantly higher than that in the control group (P<0.01), while the degree of damage in the PA group was lower than that in the ISO group (P<0.05). Conclusion Endothelin is involved in the pathologic process of experimental myocardial injury in rats. Phosphoramidon can protect the myocardial injury in rats and its mechanism may be to inhibit ECE and reduce the endothelins production.