白细胞介素4对受照小鼠小肠粘膜免疫功能的促恢复作用

RECOVERY OF SMALL EVTESTBVAL MUCOSAL IMMUNE FUNCTION BY INTERLEUKIN-4 IN IRRADIATED-MICE

采用光镜、免疫酶联吸附实验、免疫组化、逆转录聚合酶链式反应(RT-PCR )等方法,观察了小鼠受8Gy^(60)Coγ射线全身1次性照射后,肠道粘膜免疫功能损伤机理及其调控的措施。结果表明,射线可直接损伤肠道淋巴细胞,导致肠道固有层中分泌免疫球蛋白A的浆细胞数量减少。同时,射线还能抑制肠道淋巴组织中调控免疫球蛋白M型B淋巴细胞向免疫球蛋白A型B淋巴细胞转化的白细胞介素-4基因表达,使分泌免疫球蛋白A的细胞进一步减少,从而使小肠粘液中分泌性免疫球蛋白A含量明显减少。用重组人白细胞介素4调控后,肠道固有层中浆细胞的数量明显增加,提高粘液中分泌性免疫球蛋白A的含量,对放射损伤后肠道粘膜免疫功能的恢复有一定防治作用。

Light microscopy ,immunohistochemistry, ELISA and RT-PCR were used to explore the mechanism and regulated methods of small intestine mucosal immunity dysfunction in mice after 8Gy irradiation of 60Co γ-rays. Results show that the irradiation could directly injure intestine lymphocytes and inhibit the IL-4 mRNA expression of mesenteric lymphocytes, with the numbers of IgA secreting plasma cells and the quantity of mucous slgA in the intestine being significantly reduced. It has been found that rhIL-4 could increase the number of IgA-secreting plasma cells in the lamina propria and quantity of mucous slgA. This suggests that rhIL-4 could be of help for recovery of the function of intestinal mucosal immunity.