CsA滴眼液防治角膜缘移植排斥反应的实验研究

The study of CsA eye drops on preventing and treating immune rejection on limbal allograft transplantation

目的 探讨环孢霉素A(CsA)滴眼液对同种异体角膜缘移植术后的免疫抑制作用。方法 32只新西兰白兔随机分成CsA组和生理盐水对照组,每组16只。建立右眼角膜缘缺陷症动物模型,1月后实施同种异体角膜缘移植术,术后分别给予1％CsA滴眼液及生理盐水治疗,共4周。移植术后每日观察植片存活、角膜新生血管和上皮再生情况,共8周;术前1日及术后1～8周动态监测外周血T淋巴细胞CD25的表达。结果 对照组先于CsA组出现上皮排斥反应(P<0.05)。CsA组外周血T淋巴细胞CD25表达低于对照组(P<0.05)。结论 同种异体角膜缘移植术后早期应用CsA滴眼液可抑制外周血T淋巴细胞CD25的表达从而抑制排斥反应发生。

Objective To discuss the effects of the immunosuppression of CsA drops on limbal allograft translpantation. Methods Limbal deficiency models were established in 32 New Zealand rabbits, which were randomized into two groups, each group had 16 rabbits. Limbal allograft transplantation was adopted one month later. After transplantation, CsA group and control group were treated by 1% CsA eye drops and physiological saline respectively. Grafts survival and ocular surface were observed clinically for 8 weeks. The expression of CD25 in peripheral T cells was identified dynamically before and after transplantation 1 to 8 weeks. Results Epithelium rejection appeared firstly in control group (P<0.05),which had higher level of the expression of CD25 in peripheral T cells (P<0.05). Conclusion Topically administered CsA eye drops early after limbal allograft transplantation can delay allograft rejection by inhibiting effectively the expression of CD 25 in peripheral T cells.