汉族人载脂蛋白B基因3′端可变数目串联重复序列与冠心病的关系研究

Correlation of 3′ variable number of tandem repeats of apolipoprotein B gene and coronary heart disease in Han nationality

目的 探讨汉族人载脂蛋白 (apo)B基因 3′端可变数目串联重复序列 (3′VNTR)多态性与冠心病的关系。方法 应用聚合酶链反应结合琼脂糖凝胶电泳技术检测了 137例冠心病患者和12 0例健康对照组apoB基因 3′VNTR多态性基因型 ,探讨了apoB基因多态性对血脂水平的影响。结果 冠心病组 3′VNTR B(大等位基因 )频率明显高于对照组 (10 95 %比 5 0 0 % ,P <0 0 5 ) ,且与性别、家族史、吸烟史、体重指数及冠状动脉病变程度无明显关系。冠心病组中 3′VNTR B等位基因携带者血清总胆固醇 (TC)明显高于不含 3′VNTR B等位基因者 ,而高密度脂蛋白胆固醇 (HDL C)明显低于不含 3′VNTR B等位基因者。Logistic回归分析显示 ,年龄、吸烟、家族史、3′VNTR、TC、甘油三酯 (TG)和低密度脂蛋白胆固醇 (LDL C)是冠心病发生的独立危险因素。结论 apoB基因 3′VNTR多态性对人群血脂水平有影响 ,3′VNTR B等位基因可能与汉族人冠心病的发生相关联。

Objective To investigate the correlation between 3′ variable number of tandem repeats (VNTR) of apoB gene polymorphisms and coronary heart disease (CHD) in Han nationality. Methods Using PCR-gel electrophoresis, we analyzed the genotypes of apoB gene 3′VNTR in 120 healthy individuals and 137 patients with evidence of CHD.The association of gene polymorphisms of apoB and levels of serum lipids, lipoproteins, apolipoproteins were also studied. Results Totally 12 segregating alleles and 32 genotypes of 3′VNTR of the apoB gene were characterized. The results showed that the frequency of 3′VNTR-B big alleles (HVE≥38) in CHD cases was higher than controls(10.95% vs 5.00%, P <0.05). We also found that there were no relationship between gender, family history of CHD, smoking, BMI and degree of coronary artery lesions and 3′VNTR polymorphism of the apoB gene. In the CHD cases, individuals with 3′VNTR-B allele had significantly higher levels of TC and lower levels of HDL-C than those without 3′VNTR-B allele. These findings were also present in the control group, but did not reach a level of significance. Spearman correlation analysis showed that 3′VNTR-B allele was dependently related to TC levels( P <0.05). Moreover, we found that age, smoking, family history of CHD, apoB 3′VNTR, TC, TG and LDL-C were the independently risk factors of CHD analysed by logistic regression. Conclusion 3′VNTR polymorphisms of the apoB gene affect the levels of serum lipids and lipoproteins. It is suggested that 3′VNTR-B alleles may be correlated with CHD in Han nationality from northern China.