卷须链霉菌木聚糖酶B的纯化

Purification of XynB from Streptomyces cirratus

为了进一步研究链霉菌木聚糖酶的酶学性质 ,探讨了卷须链霉菌一种木聚糖酶的纯化方法。粗酶液经硫酸铵饱和度为 2 0 %～ 5 0 %的分级沉淀和Q sephroseFF离子交换柱层析 2步纯化 ,得到一种电泳纯的木聚糖酶 ,定名为XynB ;酶蛋白纯化倍数达到 10 0倍 ,酶活力回收率达到 2 4 0 %。SDS PAGE结果表明 ,该木聚糖酶的分子质量为 2 2 0ku ,属于低分子质量的木聚糖酶。

To study further the property of xylanases from Streptomyces, purification of a xylanase from Streptomyces cirratus D-10 was investigated. A xylanase, named XynB, was purified by stepwise ammonium sulphate precipitation (20%-50% saturation) and Q-sephrose FF ion exchange chromatography. After two-step treatment, ten-fold purification was obtained with 24.0% activity recovery. The purified XynB showed as a single protein band on SDS-PAGE that molecular weight of the enzyme was estimated to be 22.0 ku by SDS-PAGE, and belonged to one of lower molecular weight xylanases.