胃癌细胞鸡胚移植模型中血清基质金属蛋白酶9检测意义

The Significance of MMP-9 Examination in Serum from Embryo of Gastric Cancer Model

目的 :测定胃癌细胞接种至鸡胚绒毛尿囊膜后的成瘤过程中鸡胚血清基质金属蛋白酶 9( MMP- 9)含量变化。方法 :利用形态学方法观察胃癌细胞接种至鸡胚绒毛尿囊膜后的成瘤过程 ,并通过酶联免疫吸附测定 ( ELISA)法检测不同时间点鸡胚模型血清中 MMP- 9含量的变化 ,并结合胃癌生物学行为进行分析。结果 :1× 1 0 6·ml-1 胃癌细胞组于接种 72 h后 ,出现肉眼可见独立结节 ,随时间推移 ,结节体积增大 ,且鸡胚绒毛尿囊膜上较大血管向结节处集中 ;1× 1 0 6·ml-1胃癌细胞组 ,72 h后血清 MMP- 9含量明显增高 ,至 7d时达到最高值。结论 :胃癌细胞的成瘤过程中 MMP- 9水解酶含量明显增加 ,此模型用于胃癌特性的研究是可行的。

Objective:To investigate the changes matrix metalloproteinase 9 (MMP 9) content from sera of chick embryoes during the progression of transformed models of gastric cancer cells on chorioallantolic membrane (CAM).Methods:Morphometric investigation method was used to study the tumor generation on CAM; Enzyme linked immunosorbent assay (ELISA) method was used to test MMP 9 concentrations in chick embryos′sera transferred by cancer cells at different points of time; the relationship between MMP 9 and cancer biological characteristics was analyzed.Results:In the group of 1×10 6·ml -1 gastric cancer cells, a single clot which could be seen by naked eyes appeared at 72 hours after inoculation. With the time going on, the volumes of the clot became larger and larger, and the neovessels on CAM accumulated to the clot. In the group of 1×10 6·ml -1 gastric cancer cells, the MMP 9 content in sera extremely increased at 72 hours after inoculation and increased continuously till the maxim at 7 days after inoculation. Conclusion:The whole progression of cancer development is accompanied with the increase of MMP 9 concentration. This model is feasible to study the characteristics of gastric cancer.