基质金属蛋白酶及其抑制剂在乳腺癌中的表达及意义

Significance of Expression of Matrix Metalloproteinases and Their Inhibitors in Breast Carcinoma

目的 :探讨乳腺癌组织中基质金属蛋白酶 2 ( MMP- 2 )、基质金属蛋白酶 9( MMP- 9)及金属蛋白酶 1组织抑制剂 ( TIMP- 1 )、金属蛋白酶 2组织抑制剂 ( TIMP- 2 )的表达及它们与乳腺癌生物学行为的关系。方法 :应用免疫组织化学 SP法 ,检测 49例乳腺癌、1 0例癌旁正常组织中MMP- 2、MMP- 9、TIMP- 1及 TIMP- 2的表达及分布。结果 :乳腺癌组织中 MMP- 2、MMP- 9、TIMP- 1及 TIMP- 2的表达明显高于癌旁正常组织 ,二者之间差异具有显著性 ( P<0 .0 1 ) ;乳腺癌组织中 MMP- 2、MMP- 9及 TIMP- 1主要在肿瘤细胞胞质内表达 ,而 TIMP- 2表达于瘤细胞和间质细胞胞质 ;乳腺癌组织中 MMP- 2、MMP- 9的表达与组织学分级、淋巴结转移密切相关( P<0 .0 5 ) ,能促进乳腺癌的侵袭和转移 ,而 TIMP- 1、TIMP- 2的表达与肿瘤生物学行为无相关性( P>0 .0 5 )。结论

Objective: To study on the relationship between the expression of matrix metalloproteinase 2 (MMP 2) ,matrix metalloproteinase 9(MMP 9) and tissue inhibitor of metalloproteinases (TIMP 1 and TIMP 2) and biological behaviors in breast carcinoma. Methods:The expression and distribution of MMP 2, MMP 9,TIMP 1 and TIMP 2 were detected in 49 cases of breast carcinoma and 10 cases of corresponding non tumor normal tissues by immunohistochemical technique as a control. Results:In comparison with corresponding non tumor normal tissue group, the expression of MMP 2,MMP 9, TIMP 1 and TIMP 2 in breast carcinoma group was significantly higher ( P <0.01). MMP 2, MMP 9, and TIMP 1 were expressed predominantly in tumor cytoplasm, but positive staining of TIMP 2 was seen in the tumor and stroma cytoplasm. The expressions of MMP 2 and MMP 9 were correlated with histological grade and lymph node metastasis ( P <0.05), MMPs may promote tumor invasion and metastasis. There was no relationship between the expression of TIMP 1 or TIMP 2 and tumor biological behavior. Conclusion:MMPs may be a reliable indicator to determine biological behaviors and clinical therapy of breast carcinoma.