大鼠肝移植术后一氧化氮和白细胞介素2的变化

Changes in NO and IL-2 after Liver Transplantation of Rats

目的 :探讨一氧化氮 ( NO)在 BN至 Lew大鼠异种原位肝移植急性排斥反应中的价值。方法 :应用 BN至 Lew近交系大鼠异种原位肝移植急性排斥反应及同基因 ( Lew- Lew)肝移植动物模型 ,检测肝移植术后血浆 NO和白细胞介素 2 ( IL- 2 )的变化。结果 :大鼠异种原位肝移植急性排斥反应时血浆 NO含量明显升高 ,且变化早于 IL - 2 ;FK5 0 6显著抑制 NO的合成 ,氨基胍减轻排斥反应的程度 ;同基因组 NO不升高。结论

Objective:To explore the effects of NO in the process of acute rejection. Methods:BN to Lew rat orthotopic liver transplanatation models were established to detect the changes of NO and IL 2 in liver tissues in acute rejection.The influences between aminoguanidine/FK506 and the acute rejection were studied. Results:The level of NO increased remarkblely in the process of acute rejection. FK506 inhibited the synthesis of NO significantly, aminoguanidine reduced the degree of rejection; the level of NO in Lew Lew group didn′t change. Conclusion: The level of NO can be a diagnostic index for acute rejection in liever transplantation rats.