重型肝炎患者fgl2凝血酶原酶基因的表达

Expression of fgl2 Prothrombinase Gene in Patients with Fulminant Hepatitis

目的 探讨重型肝炎患者外周血单个核细胞 (PBMNC)中fgl2凝血酶原酶mRNA的变化及其意义。方法 选择重型肝炎患者 18例 ,非重型急性肝炎 15例、非重型慢性肝炎 14例 ,应用RT PCR方法对其外周血fgl2凝血酶原酶mRNA进行半定量检测。结果 重型肝炎组外周血fgl2凝血酶原酶mRNA与 β actinmRNA表达灰度值比值为 1 55±0 2 3 ,明显高于非重型急性肝炎组 (1 3 4± 0 18)、非重型慢性肝炎组 (1 2 9± 0 16)及正常对照组 (1 3 1± 0 12 ) ,均为P <0 0 1。结论 重型肝炎患者的肝坏死可能与fgl2凝血酶原酶的高表达有关 。

Objective To investigate the changes of fgl 2 prothrombinase mRNA in peripheral mononuclear cells in patients with fulminant h apatitis and its clinical significance. Methods fgl2 prothrombinase mRNA was detected by semi-quantita tive RT-PCR in 18 cases of fulminant hepatitis, 15 cases of acute hepatitis an d 14 cases of chronic hepatitis. Results The level of fgl2 prothrombinase mRNA in the patients w ith fulminant hepatitis (1 55±0 23) was significantly higher than that in t hose with non-fulminant acute hapatitis (1 34±0 18), non-fulminant chron ic hapatitis(1 29±0 16) and normal control group(1 31±0 12)(all P <0 01). Conclution The hepaetic necrosis in patients with ful minant hepatitis may be related to the high expression of fgl2 prothrombinase, which provides a new clew for the diagnosis and treatment of severe hepatitis.