羟氯喹对佐剂关节炎大鼠滑膜肿瘤坏死因子-α表达的影响

Effect of Hydroxychloroquine on the Expr ession of Tumor Necrosis Factor-alpha in Synovial Tissue of Rats with Adjuvant Arthritis

目的 探讨羟氯喹 (HCQ)对佐剂关节炎 (AA)大鼠滑膜肿瘤坏死因子 α (TNF α)表达的影响。方法 建立AA大鼠模型 ,并给予HCQ等治疗 5个月 ,动态观测AA大鼠踝关节病理改变 ,并以免疫组化法检测滑膜组织中TNF α的表达。结果 治疗 8周后泼尼松组及HCQ组关节病理积分均比AA组明显减低 (P <0 0 1) ,TNF α表达水平明显降低 ,与AA组及萘普生组相比差异有显著性意义 (P <0 0 1)。结论 HCQ可明显抑制滑膜组织中TNF α的表达 。

Objective To investigate the effect of hydr ox ychloroquine(HCQ)on the expression of tumor necrosis factor-alpha (TNF-α) i n synovial tissue of rats with adjuvant arthritis. Methods Adjuvant arthritis(AA)was produced in Wistar rats by inoculating complete Freund's adjuvant (CFA). The rats were fed with HCQ and nap roxen (HCQ group), prednisone and naproxen (prednisone group) or naproxen (napro xen group) respectively. The arthral histopathologic changes were observed and t he expression of TNF-α protein in synovial tissue detected by histoimmunochemi stry at different stages after CFA inoculation. Results After 8 weeks, the signs of HCQ group and prednisone gr oup were ameliorated dramatically, and the expression level of TNF-α protein s ignificantly decreased as compared with those of AA group and naproxen group (al l P<0 01). Concolusion The combination of hydroxychloroquine and naproxe n can reduce the expression of TNF-α protein in the synovium of AA rats and al leviate the inflammatory response.