一氧化氮合酶在门脉高压脾脏及脾静脉中的分布

NOS Distribution in the Spleen and Spenic Vein of Portal Hypertension

目的 探讨一氧化氮 (NO)在门脉高压发病机制中的作用。方法 运用还原型辅酶Ⅱ 黄递酶 (NADPH d)组织化学法对门脉高压患者及动物模型的脾脏及脾静脉中一氧化氮合酶 (nitricoxidesynthase ,NOS)进行定位研究 ,并以正常大鼠及外伤性脾破裂脾切除患者作为对照。结果 门脉高压组脾脏及脾静脉内NOS阳性细胞较对照组明显增多 ,染色增强 ,计算机图像半定量分析示两组灰度及光度值具显著性差异 (P <0 0 1)。

Objective To investigate the possible role of nitric oxide (NO) in the pathogenesis of portal hypertension. Methods The distribution of nitric oxide synthase (NOS) in the spleen and splenic vein in the patients and rats with portal hypertension was st udied by NADPH-d histochemical staining. The normal rats and the patients with traumatic splenic rupture undergoing splenectomy served as control group. Results NOS-containing cells were more abundant in distribution and richer in color in spleen and splenic vein of portal hypertension than those in the control group. There were significant differences between the two groups in the parameters of area, lightness and gray. Conclusion NO may play an important role in the pathogenesis of portal hypertension.