RhD阴性血液的冻融及临床应用

Thawing and medical usage of freezing-stored RhD negative blood

目的 :探讨RhD阴性血液的冻存及复融的效果和输注后的临床疗效。方法 :对冻存的RhD阴性红细胞利用M115红细胞洗涤系统解冻去甘油洗涤 ,并比较冻存前和洗涤后红细胞的总量、平均体积、平均血红蛋白含量、最大分布宽度、游离血红蛋白含量、甘油残余量、2 ,3 二磷酸甘油酸 ( 2 ,3 DPG)含量等指标 ,同时观察临床疗效。结果 :利用M115红细胞洗涤系统对冻存的RhD阴性红细胞进行解冻去甘油洗涤 ,除红细胞收率 ( 76 3 4% )略低于国家标准 ( 80 % ) ,其它项目均符合要求 ,临床效果明显。结论 :利用M115红细胞洗涤系统对冻存的RhD阴性红细胞进行解冻去甘油洗涤的方法是稳定而可靠的 ,且临床输注后疗效显著 ,无明显的输血不良反应。

Objective: To investigate the effects of freezing-storage and thawing and medical effects of RhD negative blood after transfusion. Methods: Using M115 red blood cell washing system to wash glycerol of freezing RhD negative red blood cell, and comparing parameters of red blood cell(RBC)?mean corpusclar volume(MCV)?red blood cell volume distribution(RDW)?mean corpuscular hemoglobin(MCH)?2,3-DPG, contents of free haemoglobin, residual contents of glycerol before freezing and after thawing and medical effects.Results: Using M115 red blood cell washing system to wash glycerol of freezing RhD negative red blood cell, all parameters were up to the standard except RBC(76.34% vs 80% of standard), medical effects was significant.Conclusion: The method of washing glycerol of freezing storage of RhD negative blood is stable and reliable; the medical effect is significant after transfusion and no significant negative effects after transfusion.