反相高效液相色谱法测定牛葛口服液中葛根素含量

Determination of Puerarin in Niuge Oral Liquid with RP-HPLC

目的 :建立牛葛口服液中葛根素的含量测定方法。方法 :用Nova pakC1 8色谱柱 (3 .9mm× 15 0 .0mm ,4μm)为固定相 ,甲醇 水 =2 5∶75 (V V)为流动相 ,检测波长为 2 5 0nm。 结果 :葛根素在 0 .40 4～ 2 .0 2 0 μg范围内呈良好的线性关系 ,r =0 .9999;葛根素的加样回收率为 99.2 3 % ,RSD =1.2 9% ;连续进样的精密度RSD =0 .2 6%。结论 :该方法能消除其他成分的干扰 ,准确可靠、重现性好 。

Objective:To set up a method for the determination of puerarin in niuge oral liquid with high perfomance liquid chromatography (HPLC). Methods:A Nova Pak C 18 chromatographic column(3.9 mm×150.0 mm, 4 μm) served as the stationary phase, while a mixture of methanol H 2O=25∶75( V/V ), as the mobile phase. The flow rate was 0.7 mL·min 1 and the wavelength for detection was 250 nm. Results:A good linear correlation was shown when the concentration of puerarin lay within a range of 0.404-2.020 μg, r =0.999 9. The average rate of recovery was 99.23%, RSD =1.29%; and the RSD value of precision for continuous sample mountings was 0.26%. Conclusion:Interference of other components could eliminated by this method, which was shown to be accurate, reliable, simple and convenient with good reprodu cibility.