云南滇西高原粳稻区白叶枯病菌遗传多样性初探

Genetic Diversity of Xanthomonas oryzae pv. Oryzae from the West of Yunnan Japonica Rice Planted Area

用IS-PCR和rep-PCR法分析了17个采自云南滇西高原粳稻区的水稻白叶枯病菌(Xanthomonasoryzaepv.O-ryzae)和7个其他地区的水稻白叶枯病菌的群体遗传多样性。用2个适合中国水稻白叶枯病菌的引物J3和ERIC对这24个菌系基因组DNA进行PCR扩增反应,J3引物有14条条带,ERIC引物有13条条带,2个引物呈现18种谱型。用PHYLIPVersion3.6软件UPGMA聚类分析法分析白叶枯病菌系之间的遗传距离,可将24个病原菌分为4簇,3个来自菲律宾的白叶枯病菌与中国的菌系距离较远,云南高原粳稻区分离的菌系十分复杂,4个簇中都有,但主要集中在2个簇中,共有14个菌系。

IS PCR and rep PCR were used to analyze the genetic diversity in a population of 17 strains of Xanthomonas oryzae pv. Oryzae collected from the west of Yunnan japonica rice planted area and 7 strains from other areas. Genomic DNA from 24 strains were amplified with 2 specific primers J3 and ERIC by PCR and revealed 18 haplotypes, 14 bands by J3 primer and 13 bands by ERIC primer. Dendrograms were generated from the data sets obtained by using PHYLIP Version 3.6 UPGMA analysis. 21 strains from China were grouped in 4 clusters, 3 strains from the Philippines was out of 4 clusters. Among 17 strains from the west of Yunnan, 14 strains mainly grouped in 2 clusters.