阿霉素眶下神经注射后对兔三叉神经节的影响

The Effect of Adriamycin to the Trigeminal Ganglion Following Injection into the Rabbits Infraorbital Nerve

目的观察阿霉素对兔眶下神经及三叉神经节的作用。方法将不同浓度的阿霉素20μl注入眶下神经。从三叉神经复合动作电位、阿霉素自体荧光和形态学3个方面分不同时期对眶下神经、三叉神经节的病理生理改变予以观察。结果在适当浓度阿霉素作用下,阿霉素自体荧光于注射后10h出现,20h后达到高峰。复合动作电位振幅10h后开始降低,20h后基本消失。6个月后观察无恢复。术后15d始,可观察到病理改变,随时间延长而加重。6个月后无神经细胞再生表现。坏死神经和三叉神经节周围组织未见形态学改变。结论阿霉素可引起神经轴突和与其对应的神经元的永久损害。其作用有高度的选择性和自限性。

Objective To observe the effect of adriamycin to suborbital nerve and trigeminal ganglion in the rabbits.Methods20μl adriamycin with different concentrations of0.5%,1.0%,3.0%,5.0%were injected into the suborbital nerve trunks,respectively.The changes of compound action potential(CAP),autofluorescence and histological feature in suborbital nerve and trigeminal ganglion were observed.Results With suitable adriamycin concentration,autofluorescence could be observed at10hours after injection,and reached highest at20hours.CAP amplitudes reduced at10h,disap-peared after20h and could not be stimulated after6months.Morphological change began after15d and became obvious with time going.The regeneration of neurons was not observed6months after injection,while the tissue surrounding the necrotic nerve and ganglion cells were normal morphologically.Conclusions Adriamycin can damage axons and corre-sponding ganglion cells by the way of suicide transport permanently,but the damage was highly selective and self-limited.