基质金属蛋白酶组织抑制物-1在宫颈癌的表达与临床意义

Expression of Tissue Inhibitor of Metalloproteinase-1 and Its Clinical Significance in Invasive Carcinoma of Cervix

目的 :探讨基质金属蛋白酶组织抑制物 - 1(TIMP- 1)在早期宫颈癌的表达和临床意义。方法 :采用免疫组织化学 SP法检测 75例早期宫颈浸润癌 (宫颈癌组 )、18例宫颈上皮内瘤样病变 (CIN组 )和 15例癌旁正常宫颈上皮 (对照组 )中 TIMP- 1的表达情况 ,并检测其中微血管密度 (CD34 标记 )和癌细胞增殖标记指数 (Ki- 6 7标记 )。结果 :TIMP- 1主要表达于宫颈癌细胞膜和 (或 )细胞浆 ,Ki- 6 7主要表达于癌细胞核 ,CD34 主要表达于癌巢间质血管内皮细胞。从对照组到 CIN组再到宫颈癌组 ,TIMP- 1的阳性表达率未见显著升高 (P>0 .0 5 )。 TIMP- 1在宫颈癌中表达与 FIGO分期、组织学分级、盆腔淋巴结转移和间质浸润深度有关 (P<0 .0 5 ) ;而与年龄、组织学类型、脉管浸润、Ki- 6 7表达及微血管密度无关 (P>0 .0 5 )。 FIGO分期为 期、组织学分级为 级、有盆腔淋巴结转移及突破深肌层间质浸润者 ,其 TIMP- 1阳性表达率分别显著低于 FIGO分期为 期、组织学分级在 级以内、无盆腔淋巴结转移和浸润深度在浅肌层间质以内者 (P<0 .0 5 )。结论 :宫颈癌 TIMP- 1表达降低可能在侵袭转移中起一定作用。检测宫颈癌 TIMP- 1表达对进一步了解宫颈癌生物学行为和判断预后有一定价值。

Objective:Expression and clinical significance of tissue inhibitor of metalloproteinase-1 (TIMP-1) in early invasive carcinoma of cervix were investigated to explore its clinical significance.Methods:The expression of TIMP-1, microvessel density labeled by CD 34and proliferation index of cancer cells labeled by Ki-67 in 75 cases of early invasive carcinoma of cervix (ICC group), 18 cases of cervical intraepithelial neoplasm (CIN group) and 15 cases of normal cervical epithelium (control group) remote from tumor were detected by immunohistochemistry SP method.Results:TIMP-1 was mainly expressed in the cellular membrane and/or cytoplasm in tumor cells, whereas expression of Ki-67 was mainly confined to the nuclei, and that of CD 34 to the vascular epithelial cells in tumor stroma. The positive rates of expression of TIMP-1 did not increase remarkably from control to CIN, and then to ICC group (P>0.05). In ICC group, the expression of TIMP-1 correlated with FIGO staging, histological grading, pelvic lymph node metastasis and stroma infiltration (P<0.05), but not correlated with patients age, histological types, intravascular infiltration, Ki-67 expression and microvessel density (P>0.05). In the cases with FIGO staging Ⅱ, histological grading Ⅲ, pelvic lymph node metastasis and deeper stroma infiltration, the positive rate of expression of TIMP-1 was significantly lower than that in those without the conditions mentioned above (P<0.05).Conclusion:Lower expression of TIMP-1 may play an important role during cancer invasion and metastasis in invasive carcinoma of cervix. Detection expression of TIMP-1 may be of value in further understanding the biological behavior and predicting the prognosis of cervical carcinoma.