BCPT对CUS大鼠大脑皮质BDNF、Bax及海马超微结构的影响

Effects of BCPT on the expressions of cerebral cortex BDNF and Bax and the ultrastructure of hippocampus of rats treated with chronic unpredictable stress

目的观察细脚拟青霉代谢物提取物(BCPT)对慢性应激抑郁模型大鼠行为、海马组织超微结构、大脑皮质脑源性神经营养因子(BDNF)和Bax的影响。方法采用慢性不可预见性应激法(CUS)造成大鼠抑郁模型;采用Openfield法观测大鼠行为;采用免疫组织化学的方法对BDNF和Bax进行染色,并用图像分析仪进行半定量分析;用电镜观察海马超微结构的变化情况。结果慢性应激抑郁模型大鼠水平和垂直运动次数明显减少,BDNF阳性神经元数目明显减少,Bax阳性神经元数目明显增加,电镜显示海马超微结构出现病理变化,而BCPT对其有明显的保护作用。结论BCPT具有抗抑郁作用,增加BDNF表达、降低Bax表达、改善海马超微结构的病理变化有关。

Aim Exploration of the effects of bioactive compound from paecilomyces tenuipes (BCPT) on the behavior, ultrastructure of hippocampus, and expressions of BDNF and Bax of cerebral cortex in CUS rats. Methods The depression model rat was induced by chronic unpredictable stress(CUS). The Open field test was used to observe the behavior of CUS rats. The expressions of BDNF and Bax of cerebral cortex were studied using immunohistochemical method, and average optical density of cortical neuron was calculated by image analysis. Morphology of hippocampus was observed by transmission electron microscope. Results Compared with model group, BCPT could obviously increase the activity of ambulation and rearing and the number of positive neuron of BDNF, decrease the number of positive neuron of Bax, and effectively prevent pathological damage induced by chronic unpredictable stress. Conclusion BCPT may exhibit the effects of antidepression, up-regulate the expression of BDNF, down-regulate the expression of Bax, and improve of the ultrastructure of hippocampus.