摘要
目的探讨慢性应激大鼠海马CA3区锥体细胞内微管相关蛋白2(MAP2)表达变化及噻萘普汀的效应。方法采用强迫游泳作为应激模型,25只大鼠随机分为对照组、应激组及应激给药组。利用免疫组织化学方法及计算机图像分析技术,定量测定各组大鼠海马CA3区锥体细胞磷酸化MAP2表达水平及阳性细胞数。结果与对照组(149.34±1.81)比较,应激组大鼠海马CA3区锥体细胞磷酸化MAP2表达的平均灰度值(144.99±4.40)明显下降,给药组(148.84±2.73)则明显高于应激组;应激组阳性表达的细胞数(40.36±1.35)明显少于对照组(42.73±1.56),给药组(42.14±1.62)则明显多于应激组。结论噻萘普汀可抑制慢性应激所致的大鼠海马CA3区锥体细胞内磷酸化MAP2表达上调。
Aim To investigate changes of MAP2 expression level in rat hippocampal pyramidal cells induced by chronic stress, and to explore effects of tianeptine on them. Methods 25 rats were divided randomly into three groups:Control group,Stress group and Stree-tianeptine group. The forced-swimming was performed to rats in stress group and stress-tianeptine. Using the immunohistochemistry and the computerized image technique, expression levels of phosphorated MAP2 and the number the Positive cells were assayed quantitatively in each group. Results Compared with control group (149.34±1.81), the phosphorated MAP2 average gray degree in pyramidal cells of stress group (144.99±4.40) was significantly lower, that of the stress-tianeptine group (148.84±2.73) was significantly higher than that of stress group; The number of phosphorated MAP2 positive cells in stress group (40.36±1.35) was significantly less compared withthat of control group (42.73±1.56); that of stress-tianeptine group (42.14±1.62) was significantly more than that of stress group. Conclusion It is suggested that tianeptine could inhibit the enhancement of phosphorated MAP2 expression in hippocampal pyramidal cells induced by chronic stress.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2007年第7期938-941,共4页
Chinese Pharmacological Bulletin
基金
广东省医学科研基金资助项目(NoB2006020)
