三七总皂苷对抗H_2O_2所致大鼠脑微血管内皮细胞损伤的物质基础研究

Protective effect study of major constituent in total panax notoginsenoside on rat brain microvessel endothelial cell (RBMEC) damage induced by H_2O_2

目的研究三七总皂苷对H2O2致大鼠脑微血管内皮细胞损伤产生保护作用的物质基础。方法分离并培养大鼠脑微血管内皮细胞(RBMEC),以MTT和LDH的释放为指标,观察三七总皂苷(TPNS)、三七皂苷R1、人参皂苷Rg1、Rd、Re和Rb1对RBMEC损伤的保护作用。结果500μmol·L-1的H2O2对原代培养的RBMEC产生明显的损伤。20～200mg·L-1的TPNS,16·0μmol·L-1的R1,37·5～75·0μmol·L-1的Rg1,8·0～16·0μmol·L-1的Rd及5·4～54·0μmol·L-1的Rb1对H2O2致RBMEC的损伤具有明显的保护作用(P<0·05或P<0·01),Re则无此作用。结论TPNS对H2O2所致原代培养的RBMEC损伤具有明显的保护作用,产生这一作用的主要物质基础可能为Rb1、Rg1和Rd。

Aim To investigate the protective effect of major constituents in total panax notoginsenoside(TPNS) on rat brain microvessel endothelial cell(RBMEC) damage induced by H2O2.Methods RBMEC was successfully isolated and primarily cultured.The protective effect of TPNS,R1,Rg1,Rd,Re and Rb1 on damaged RBMECs was investigated by means of MTT and lactic dehydrogenase(LDH) in the layers of RBMEC.Results H2O2 damaged RBMEC markedly at the concentration of 500 μmol·L-1.The results of MTT and LDH showed markedly protective effect on damaged RBMEC at the concentration of 20～200 mg·L-1 for TPNS,16.0 μmol·L-1 for R1,37.5～75.0 μmol·L-1 for Rg1,8.0～16.0 μmol·L-1 for Rd and 5.4～54.0 μmol·L-1 for Rb1 respectively(P<0.05 or P<0.01).Re showed no markedly protec-tive effect.Conclusion TPNS showed markedly protective effect on primarily cultured RBMEC damage induced by H2O2.Ginsenoside Rb1,Rg1 and Rd are probably the major constituents in TPNS which play important role in the brain protection.