摘要
目的探究循环肿瘤细胞在可切除食管鳞癌患者中的表达水平及其临床意义。方法选取36例经手术治疗的食管鳞癌患者,采用FR-CTC检测方法检测患者术前、术后外周静脉血中CTC水平,分析CTC水平与其他临床病理资料的关系,通过比较术前术后外周血CTC水平的变化,探究手术对CTC水平的影响。结果本研究共入组36名患者,其中男性32人,女性4人,采集术前外周血标本36例,CTC检测结果 2.25~27.5 Unit/3 m L,采集术后标本19例,CTC检测结果 2.33~42.41 Unit/3 m L,接受三切口食管癌根治术的病人术前CTC水平显著低于两切口和左开胸病人(P=0.02),术后病理N0的患者其术前CTC水平显著高于病理N1-2的病人(P=0.01)。此外,对于无淋巴结转移的患者(23例),随着T分期的不断升高,CTC表达水平也相应升高(P=0.02)。选取了19例患者术前术后配对标本进行检测,术前术后患者CTC水平差异无统计学意义(P=0.29)。结论采用FR-CTC技术可有效检测食管鳞癌患者外周血CTC水平,术前CTC水平与肿瘤部位、病理T分期显著相关,有助于评估疾病进展程度,手术治疗并未导致肿瘤细胞进一步播散。
OBJECTIVE To investigate the expression and clinical significance of circulating tumor cells in resectable esophageal squamous cell carcinoma. METHODS 36 esophageal squamous cell carcinoma patients who received radical surgery treatment were enrolled. The CTC levels were detected by FR-CTC technique before and after surgery,and the relationship between CTC level and other clinicopathological data was analyzed. By comparing the changes of peripheral blood CTC levels before and after operation,the effect of operation on CTC level was explored. RESULTS A total of 36 patients( 32 males and 4 females) were enrolled in this study. 36 cases of preoperative specimens were collected and the results were 2.25-27.5 Unit/3 m L. 19 cases of postoperative specimens were collected and the results were 2. 33-42. 41 Unit/3 m L. The preoperative CTC levels in patients undergoing McKeown esophagogastrectomy were significantly lower than those in patients undergoing Ivor Lewis and left thoracotomy esophagogastrectomy( P = 0.02). The preoperative CTC level in patients with pathological N0 was significantly higher than that in patients with pathological N1-2( P = 0.01). In addition,in 23 patients without lymph node metastasis,the level of CTC expression increased with the increasing of T stage( P = 0. 02). We selected 19 matched specimens for examination and there was no significant difference in CTC levels before and after surgery( P = 0.29).CONCLUSION The level of CTC in peripheral blood of patients with esophageal squamous cell carcinoma can be detected effectively by FR-CTC technique. The preoperative CTC level is correlated with tumor location and pathological T stage,which is helpful to evaluate the degree of disease progression. The operation does not result in spread of tumor cells.
作者
唐继鸣
陈刚
田单
贲晓松
谢亮
周海榆
张冬坤
施瑞卿
邓澄
乔贵宾
TANG Jiming;CHEN Gang;TIAN Dan;BEN Xiaosong;XIE Liang;ZHOU Haiyu;ZHANG Dongkun;SHI Ruiqing;DENG Cheng;QIAO Guibin(Guangdong Gererwl Hospital,Guangdong Academy of Medical Sciences,Guangzhou,Guangdong 510080,China)
出处
《今日药学》
CAS
2019年第1期820-823,839,共5页
Pharmacy Today
基金
广东省省级科技计划项目(2013B021800194)
关键词
循环肿瘤细胞
食管鳞癌
手术
circulating tumor cells
esophageal squamous cell carcinoma
surgery