摘要基因敲除是一项重要的高新生物技术,在现代农业发展及医学研究领域起着重要作用。基因敲除技术从传统的同源重组、杂合性缺失(loss of heterozygosity,LOH)、体细胞打靶发展到新型基因敲除技术—锌指核酸酶技术(zinc finger nuclease,ZFN)及转录激活因子样效应核酸酶(Transcription Activator Like Effectors Nuclease,TALEN)技术等。文章综述了以上几种基因敲除技术在大中型动物上的应用进展。
1Jeffrey J.Whyte,JianguoZhao,Kevin D.Wells,Melissa S.Samuel,Kristin M.Whitworth,Eric M.Walters,M. HaroldLaughlin,Randall S.Prather.Gene targeting with zinc finger nucleases to produce cloned eGFP knockout pigs[J]. Mol. Reprod. Dev. . 2011 (1)
2Christian, Michelle,Cermak, Tomas,Doyle, Erin L,Schmidt, Clarice,Zhang, Feng,Hummel, Aaron,Bogdanove, Adam J,Voytas, Daniel F.Targeting DNA Double-Strand Breaks with TAL Effector Nucleases[J]. Genetics . 2010 (2)
3Capecchi M R.Altering the genome by homologous recombination,1989(244).
4aldman AS.Targeted homolous recombination in mammalian cells. Critical Reviews in Oncology Hematology . 1992
5Smithies O,Gregg RG,Boggs SS,et al.Insertion of DNA sequences into the human chromosomal beta-globin locus by homologous recombination. Nature . 1985
6Shao C,Stambrook P J,Tischfield J A.Mitotic recombination is suppressed by chromosomal divergence in hybrids of distantly related mouse strains. Nature Genetics . 2001
7Donna Kolber-Simonds,Liangxue Lai,Steven R. Watt,Maria Denaro,Scott Arn,Monica L. Augenstein,Jeffery Betthauser,David B. Carter,Julia L. Greenstein,Yanhong Hao,Gi-Sun Im,Zhonghua Liu,Greg D. Mell,Clifton N. Murphy,Kwang-Wook Park,August Rie.Production of α-1,3-galactosyltransferase null pigs by means of nuclear transfer with fibroblasts bearing loss of heterozygosity mutations. Proceedings of the National Academy of Sciences of the United States of America . 2004
8Fujimura Tatsuya,Takahagi Yoichi,Shigehisa Tamotsu,Nagashima Hiroshi,Miyagawa Shuji,Shirakura Ryota,Murakami Hiroshi.Production of alpha 1,3-galactosyltransferase gene-deficient pigs by somatic cell nuclear transfer: a novel selection method for gal alpha 1,3-Gal antigen-deficient cells. Molecular Reproduction and Development . 2008
9Doetschman T,Gregg RG,Maeda N,et al.Targetted correction of a mutant HPRT gene in mouse embryonic stem cells. Nature . 1987
10McCreath KJ,Howcroft J,Campbell KH,et al.Production of gene-targeted sheep by nuclear transfer from cultured somatic cells. Nature . 2000
